期刊

Chemistry - A European Journal

标题

An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase

作者

Ping Wang, Yang-Liu Xia, Li-Wei Zou, Xing-Kai Qian, Tong-Yi Dou, Qiang Jin, Shi-Yang Li, Yang Yu, Dan-Dan Wang, Qun Luo, Guang-Bo Ge, Ling Yang

摘要

A highly sensitive and selective two-photon fluorescence probe for catechol-O-methyltransferase (COMT) was developed via regioselective masking of one hydroxyl group of the substrates. The probe served as an efficient tool for biological sensing and bio-imaging of COMT in complex biological systems, as well as for high-throughput screening of COMT inhibitors. The strategy used in this study also sheds new light on the development of fluorescent probes for other conjugative enzymes.

原文链接

http://onlinelibrary.wiley.com/doi/10.1002/chem.201702637/full