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Green Synthesis of Conjugated Linoleic Acids from Plant Oils Using a Novel Synergistic Catalytic System
时间:2017-08-09 14:56:00
作品信息

期刊

Journal of Agricultural and Food Chemistry


标题

Green Synthesis of Conjugated Linoleic Acids from Plant Oils Using a Novel Synergistic Catalytic System


作者

Yu Zhang, Huaxiang Gu, Hao Shi, Fei Wang and Xun Li


摘要

A novel and efficient method has been developed for converting plant oil into a specific conjugated linoleic acid (CLA) using a synergistic biocatalytic system based on immobilizedPropionibacterium acnes isomerase (PAI) and Rhizopus oryzae lipase (ROL). PAI exhibited the greatest catalytic activity when immobilized on D301R anion-exchange resin under optimal conditions (PAI dosage of 12 410 U of PAI/g of D301R, glutaraldehyde concentration of 0.4%, and reaction conditions of pH 7.0, 25 °C, and 60 min). Up to 109 g/L trans-10,cis-12-CLA was obtained after incubation of 200 g/L sunflower oil with PAI (1659 U/g of oil) and ROL (625 mU/g of oil) at pH 7.0 and 35 °C for 36 h; the corresponding conversion ratio of linoleic acid (LA) to CLA was 90.5%. This method exhibited the highest proportion of trans-10,cis-12-CLA yet reported and is a promising method for large-scale production.


原文链接

http://pubs.acs.org/doi/10.1021/acs.jafc.7b00846

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