期刊

Chemistry - A European Journal

标题

Alternative Mechanistic Strategy for Enzyme Catalysis in a Ni-Dependent Lactate Racemase (LarA): Intermediate Destabilization by the Cofactor

作者

Xiaoyong Zhang, Prof. Dr. Lung W. Chung

摘要

The unique role of a new (SCS)Ni cofactor in lactate racemase (LarA) is examined in this computational study. The Ni-dependent cofactor could increase dehydrogenation barriers and particularly destabilize the NADH-like pyruvate intermediates, due to the lower electrophilicity of the cofactor and the ring strain in the intermediates. This intermediate destabilization by a cofactor in LarA, which can promote racemization, is uncommon in enzymatic catalysis.

原文链接

http://onlinelibrary.wiley.com/doi/10.1002/chem.201605864/full