期刊

Chem Comm

标题

An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins

作者

Kai Cai, Ying Tan, Chunyan Tan, Jiatao Wu, Pan Wu, Jiamei Liang, Shuwen Liu, Bibo Zhang, Yuyang Jiang

摘要

The iminodiacetate–Ni2+–hexahistidine system is extensively used in protein purification. In this study, an anionic conjugated polyelectrolyte (CPE) with a poly(p-phenylene ethynylene) backbone and iminodiacetate side chains, named PPEIDA, was designed and synthesized. Recognition and visualization of hexahistidine-tagged (His-tagged) proteins using PPEIDA was demonstrated

原文链接

http://pubs.rsc.org/en/content/articlelanding/2017/cc/c7cc00850c#!divAbstract