期刊

Chem Comm

标题

Single-molecule imaging reveals the stoichiometry change of β2-adrenergic receptors by a pharmacological biased ligand

作者

Yahong Sun,a Nan Li,a Mingliang Zhang,ab Wei Zhou,a Jinghe Yuan,a Rong Zhao,a Jimin Wu,b Zijian Li,b Youyi Zhang*b and Xiaohong Fang*a

摘要

The stoichiometry of the β2-adrenergic receptor (β2AR) was determined using single-molecule fluorescence imaging in living cells. The results showed that β2AR mainly existed as monomers under physiological conditions and exhibited β-arrestin-dependent dimerization upon stimulation with the pharmacological biased ligand carvedilol. The association of β2AR dimerization with biased signalling is revealed.

原文链接

http://pubs.rsc.org/en/content/articlelanding/2016/cc/c6cc90233b