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Acoustofluidic Fluorescence Activated Cell Sorter
时间:2017-02-09 10:45:00
作品信息

期刊
Analytical Chemistry

标题
Acoustofluidic Fluorescence Activated Cell Sorter

作者
Ahmad Ahsan Nawaz, Yuchao Chen, Nitesh Nama, Ruth Helmus Nissly, Liqiang Ren, Adem Ozcelik, Lin Wang, J. Philip McCoy, Stewart J. Levine, and Tony Jun Huang

摘要
Selective isolation of cell subpopulations with defined biological characteristics is crucial for many biological studies and clinical applications. In this work, we present the development of an acoustofluidic fluorescence activated cell sorting (FACS) device that simultaneously performs on-demand, high-throughput, high-resolution cell detection and sorting, integrated onto a single chip. Our acoustofluidic FACS device uses the “microfluidic drifting” technique to precisely focus cells/particles three dimensionally and achieves a flow of single-file particles/cells as they pass through a laser interrogation region. We then utilize short bursts (150 μs) of standing surface acoustic waves (SSAW) triggered by an electronic feedback system to sort fluorescently labeled particles/cells with desired biological properties. We have demonstrated continuous isolation of fluorescently labeled HeLa cells from unlabeled cells at a throughput of ∼1200 events/s with a purity reaching 92.3 ± 3.39%. Furthermore, 99.18% postsort cell viability indicates that our acoustofluidic sorting technique maintains a high integrity of cells. Therefore, our integrated acoustofluidic FACS device is demonstrated to achieve two-way cell sorting with high purity, biocompatibility, and biosafety. We believe that our device has significant potential for use as a low-cost, high-performance, portable, and user-friendly FACS instrument.

原文链接
http://pubs.acs.org/doi/10.1021/acs.analchem.5b02398

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