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Traceless protein delivery with an efficient recyclable nanocarrier
时间:2017-01-10 19:43:00
作品信息

标题

Traceless protein delivery with an efficient recyclable nanocarrier

期刊

Biomaterials Science

作者

Xuanjun Wu, Yunlong Song, Jiahuai Han, Liu Yang and  Shoufa Han

摘要

Intracellular delivery is a prerequisite for the efficacy of many pharmaceutical proteins. Herein, vitamin B6 (pyridoxal-5′-phosphate, PLP) functionalized calcium phosphate (CP) is used as the bio-recyclable nanocarrier for delivery of proteins into cells. Proteins could be loaded on/released from PLP-CP via formation/hydrolysis of pH sensitive aldimine bridging lysine and surface-displayed PLP. The loaded proteins could be delivered into the cytosol of HeLa, HepG2 and L929 cells where the carrier could be metabolized into endogenous metabolites of Ca2+, HPO42−, and vitamin B6. PLP-CP mediated cell transduction is 10–40 folds more efficient than TAT which is a widely used cell penetrating peptide, demonstrating the utility of PLP-CP as the traceless platform for high-efficiency delivery of proteins into mammalian cells.

原文链接

http://pubs.rsc.org/en/content/articlelanding/2013/bm/c3bm60128e#!divAbstract

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