Lab on a Chip
Lift-off cell lithography for cell patterning with clean background
Cong Wu, Xiongfeng Zhu, Tianxing Man, Pei-Shan Chung, Michael A. Teitell and Pei-Yu Chiou
We developed a highly efficient method for patterning cells by a novel and simple technique called lift-off cell lithography (LCL). Our approach borrows the key concept of lift-off lithography from microfabrication and utilizes a fully biocompatible process to achieve high-throughput, high-efficiency cell patterning with nearly zero background defects across a large surface area. Using LCL, we reproducibly achieved >70% patterning efficiency for both adherent and non-adherent cells with <1% defects in undesired areas.